Abstract
ANTIBACTERIAL EFFECT OF BACTERIOCIN FROM LEUCONOSTOC MESENTEROIDES SSP. CREMORIS AGAINST DIARRHEAL CAUSATIVE BACTERIA

Raghad A. Aziz, Jehan Abdul Sattar Salman* and Omar A. H. Hachim

ABSTRACT

Antibacterial activity of crude bacteriocin of Leuconostoc mesenteroides ssp. cremoris included non-concentrated and concentrated supernatant (bacteriocin) was tested against diarrheal causative bacteria Escherichia coli, Salmonella ser. paratyphi A, Shigella group. and Vibrio cholerae. Results showed that non-concentrated crude supernatant (bacteriocin) from 2 isolates [L. mesenteroides ssp. cremoris (Lc3) and L. mesenteroides ssp. cremoris (Lc4) ] had inhibitory effect with inhibition diameter ranged between (11-13) mm against E.coli, Shigella group, Salmonella ser. paratyphi A and V. cholera, while other isolates were unable to inhibit the growth of pathogenic bacteria. The concentrated crude supernatant bacteriocin showed inhibitory activity against E.coli, Shigella group, Salmonella ser. paratyphi A and V. cholerae with diameter of 19, 18, 15 and 25 mm respectively for for Leuconostoc mesenteroides ssp. cremoris (Lc3) isolate and inhibition diameter of 17, 18, 16 and 20 mm for Leuconostoc mesenteroides ssp. cremoris (Lc4) isolate.

Keywords: Leuconostoc mesenteroides,Bacteriocin, Diarrheal causative bacteria.


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