European Journal of Biomedical and Pharmaceutical Sciences

TO STUDY ANTIOXIDANT ACTIVITY OF 70% METHANOLIC EXTRACT OF TRIUMFETTA RHOMBOIDEA BY DPPH RADICAL SCAVENGING METHOD.

Baban Sukadeo Thawkar, Dr. Mohan Kale, Monica Oswal, Karishma Maniyar, Ketaki Kadam, Sumit Kamat

ABSTRACT

In this study Anti-oxidant activity was performed by Diphenylpicrylhydrazyl (DPPH) for methanolic extract of Triumfetta rhomboidea (Family: Tiliaceae). Test solutions of various concentrations were prepared by mixing methanolic extract of TR and DPPH solution and compared by taking absorbance of ascorbic acid as a reference standard solution. The reaction was carried out in triplicate and the decrease in absorbance was measured at 517nm. The methanol extract of TR shows better Antioxidant potential as compare to standard by DPPH radical scavenging method. Methanolic extract of Triumfetta rhomboidea at 100 μg/ml showed maximum 90.71±0.93 inhibition. Ascorbic acid from 5-200 μg/ml shows more than 96.89 % inhibition. IC 50 for TR and ascorbic acid was found to be 18 μg/ml and 8 μg/ml respectively. Antioxidant activity was found to be due to presence of flavonoids and phenols, however decrease in activity beyond 100 μg/ml dose.

Keywords: Triumfetta rhomboidea, Antioxidant activity, % Inhibition, DPPH Radical scavenging method, flavonoids.