European Journal of Biomedical and Pharmaceutical Sciences

COMPARATIVE STUDY BETWEEN E.COLI AND K.PNEUMONIAE ISOLATED FROM THE SAME CLINICAL SOURCE (UTI) OF BIOFILMS FORMATION AND ANTIBIOTICS RESISTANCE

Ali Hussein Alwan* and Noor Naeem Khwen

ABSTRACT

One hundred urine samples were collected from urinary tract infection patients. Ages were between two months to 72 years old. UTI infections were more common in female than male and the percentage was 76% female,24% in E.coli while 88% female,22% male in K.pneumoniae. Both species in planktonic state showed resistance to more than six antibiotics, Ciprofloxacin was the most effective antibiotic against both species. Biofilm production was carried on by two methods CRA and TCP, In CRA method 65% of E.coli and 58% of K.pneumoniae were biofilm former, 23% of E.coli and 6% of K.pneumoniae were non-biofilm former while 12% of E.coli and 36% of K.pneumoniae gave non identified results. TCP method showed that 69% of E.coli and 72% of K.pneumoniae were strong biofilm producers,6% of E.coli and 12% of K.pneumoniae were moderate producers while 25% of E.coli and 16% of K.pneumoniae were non biofilm producers. Adhesion average to epithial cells of E.coli and K.pneumoniae was 42.9% and 44% respectively. The stronger biofilm producers isolates from both species were chosen to study the antibiotic susceptibility of these isolates in biofilm state against Ciprofloxacin, Gentamycin and Amikacin in different concentrations. Conclusion: It's thought that the clinical source of isolation play a role in the ability of biofilms production and resistance to antibiotics.

Keywords: Biofilms, Adhesion, UTI, E.coli, K.pneumoniae.