STABILITY-INDICATING SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FENOTEROL HYDROBROMIDE IN ITS PURE FORM AND PHARMACEUTICAL PREPARATION.
Khalid Abdel-Salam Attia, Mohammed Wafaa Nassar, Hamed M. Abou-Seada and Samir Morshedy Mohammed Morshedy*
ABSTRACT
Three Simple, rapid, sensitive, accurate and precise stability-indicating
spectrophotometric methods was developed for the determination of
fenoterol in bulk powder and in pharmaceutical preparation. Method
(A) Ratio derivative method(1DD); is used for the determination of
intact fenoterol in presence of its degradation product at 236 nm in the
range of 2 – 14 μg ml-1 with LOD of 0.049 μg ml-1 and LOQ of 0.162
μg ml-1.Method (B) Ratio difference method ;is based on measuring
the difference in the amplitude of intact fenoterol in presence of its
degradation product at two different wavelengths ,this is done at 225 nm and 277 nm in the
range of 2 – 14 μg ml-1 with LOD of 0.050 μg ml-1 and LOQ of 0.165 μg ml-1.Method (C)
Mean centering method; the method was applied for the analysis of fenoterol in presence of
its degradation product this is done at 236 nm in the range of 2 – 14 μg ml-1 with LOD of
0.072 μg ml-1 and LOQ of 0.241 μg ml-1. The methods was validated and successfully
applied to the determination of Berotec tablets with an average percent recovery ± RSD% of
100.02 ± 0.643 for method (A), 100.32 ± 0.21 for method (B) and 100.43 ± 0.86 for method
(C).The obtained results were statistically compared with those of the reported method by
applying t-test and F-test at 95% confidence level and no significant difference was observed
regarding accuracy and precision.
Keywords: Fenoterol, Ratio derivative, Ratio difference, Mean centering, pure form, pharmaceutical preparation.
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