Abstract
ABSENCE OF HUMAN PAPILLOMAVIRUS GENOME IN OCULAR SURFACE SQUAMOUS NEOPLASIA PATIENT FROM NORTH EAST INDIA

Akshay Chandra Deka*, Ananta Madhab Dutta, Vidhya S. and Pratap Chandra Sarma

ABSTRACT

Aim - To study the probable human papilloma virus infection in Ocular surface squamous neoplasia (OSSN from North eastern part of India. Materials and method- We have retrieved histologically proven cases of OSSN formalin-fixed, paraffin-embedded tissue blocks (n = 40) and fresh tissues (n = 5) for the study. The presence of HPV was detected by polymerase chain reaction using MY09/MY11 consensus primers. SiHa and HeLa cell lines were used as positive controls for HPV analysis. Results- This study includes 31 squamous cell carcinomas and 9 conjunctival intraepithelial neoplasias. All of the cases were found to be negative for HPV using polymerase chain reaction whereas the SiHa and HeLa cell lines were positive which harbor HPV DNA. Conclusions- Sensitive, polymerase chain reaction did not show the presence of HPV DNA in OSSN. Thus, HPV appears to play no significant role in the etiology of OSSN in northeastern India.

Keywords: Human papilloma virus, CSSC, OSSN, Polymerase chain Reaction.


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