STABILITY-INDICATING SPECTROFLUORIMETRIC DETERMINATION OF DOXAZOSIN MESYLATE IN RAW MATERIAL AND PHARMACEUTICAL PREPARATION.
Khalid Abdel-Salam Attia, Mohammed Wafaa Nassar, Hamed M. Abou-Seada and Samir Morshedy Mohammed Morshedy*
ABSTRACT
A Simple, rapid, sensitive, accurate and precise spectrofluorimetric
method has been developed for selective determination of doxazosin
mesylate in presence of its alkaline degradate in bulk powder and in
pharmaceutical preparation. The proposed method is based on
measuring the native fluorescence of doxazosin in methanol at 403 nm
after excitation at 252 nm. All variables that affect fluorescence
intensity such as diluting solvents and pH were studied and optimized.
The fluorescence–concentration plot was rectilinear over the range of
10-140 ng/ml with a lower detection limit (LOD) of 0.060 ng/ml and lower quantitation limit
(LOQ) of 0.199 ng/ml. The proposed method can selectively analyze the drug in presence of
up to 71.43% of its alkaline degradate with mean recovery of 101.37±0.72. The method was
validated and successfully applied for the determination of doxazosin in its commercial
preparation with an average percent recovery ± RSD% of 100.14 ± 0.89. The obtained results
were statistically compared with those of the reported method by applying t-test and F-test at
95% confidence level and no significant difference was observed regarding accuracy and
precision.
Keywords: Stability-indicating, doxazosin, spectrofluorimetric, excitation, emission and pharmaceutical preparation.
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