European Journal of Biomedical and Pharmaceutical Sciences

EFFECT OF TOCOTRIENOL SUPPLEMENTATION ON NEUROBEHAVIORAL PARAMETERS OF RAT

Pitchaiah Dasari, Anandamurali R. and *Prasunpriya Nayak

ABSTRACT

Background: Role of free radicals is suggested in neurodegenerative diseases. Hence, antioxidants may have putative benefits against neuropathological changes. Unsaturated isoprenoid side chain provided Tocotrienol (TT), a constituent of vitamin E, better antioxidant activity. Several in vitro studies have demonstrated strong neuroprotective nature of TT and recent in vivo studies also support so. Objective: Current study is aimed to evaluate the effect of tocotrienol supplementation on the neurobehavioral parameters of rat. Materials and Method: Male albino Wistar rats were treated with tocotrienol (10 mg/day) for two weeks and maintained for four weeks (TT+) along with rats those are not exposed to TT (TT0). Spontaneous motor activity (SMA), Rota-rod performances (RRP) and open-field activity (OFA) are evaluated fortnightly starting from pretreatment phase. Performances in Morris water maze (MWM) are recorded once at the end of experiment. Data collected from both the groups are statistically processed with Kruskal-Wallis test and Mann-Whitney pairwise comparisons. Results: Significantly improved SMA score was recorded by TT+ group in 4th week. Improved RRPs were also noticed throughout the study in case of TT+ group. However, no significant difference between the TT+ and TT0 group was noted in terms of OFA and MWM experiments. Conclusion: From the results it can be suggested that the dose and duration of tocotrienol supplementation has improved some of the tested neurobehavioral performances and the impact continues at least for 4 weeks after the supplementation. With the modification of dose and duration of TT supplementation it can be used against specific neuropathologies to prevent the behavioral declension.

Keywords: Tocotrienol, Brain, Spontaneous motor activity (SMA), Rota-rod performance (RRP), Open-field activity (OFA), Morris water maze (MWM).