Abstract
A NEW VALIDATED SIMULTANEOUS RP- HPLC ASSAY METHOD FOR ESTIMATION OF TWO FLAVONES (APIGENIN AND GENKWANIN) IN API DRUGS

Maddi Sesharao* and Vallabaneni Madhavarao

ABSTRACT

A reversed-phase high-performance liquid-chromatography method has been successfully developed for the simultaneous determination of Apigenin and Genkwanin in API drugs. The RP-HPLC method employed a Symmetry C18 reversed phase column (100 × 4.6 mm×3.5μm) with an isocratic mixture of Acetonitrile and 0.1% glacial acetic acid buffer in the ratio of (45:55; v/v) as the mobile phase. The column temperature was kept at 30°C. The flow rate was 1.0 mL/min and detection was by means of a PDA detector at wavelength of 207 nm. All the active components were successfully eluted with mean retention times of 2.02 and 5.12 min for Apigenin and Genkwanin respectively. The method was found to be linear (R2> 0.99), precise (RSD NMT 2.0%), accurate (recoveries 98.0–102.0%), specific, rugged and robust. The validated method can be used in routine quality control analysis of bulk drugs without any interference by excipients.

Keywords: Apigenin, Genkwanin, RP-HPLC, Method development, Validation.


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