European Journal of Biomedical and Pharmaceutical Sciences

NEW DELHI METALLO-?-LACTAMASE 1 (NDM-1) PRODUCING ESCHERICHIA COLI IN BASRAH HOSPITALS. IRAQ

Ismaal Jmia Abas and Maytham A. Al-Hamdani*

ABSTRACT

Resistance to carbapenems is developing around the world Escherichia coli(E.coli) resistance to carbapenems is associated with production of carbapenem-hydrolyzing class B metallo-β-lactamases (MBL).New Delhi metallo-β-lactamase 1 (NDM-1) is one of the most recently discovered MBL among E.coli .The present work was aimed to study the prevalence of bla NDM-1 gene among carbapenem-resistant E.coli isolates from six hospitals in Basrah, Iraq Isolates identified and antibiotics susceptibility were assayed by using VITEK2Compact.Phenotypic detection of carbapenemase was performed using combined-disc synergy test (CDST), Chrom ID Carba agar and using RAPIDEC CARBA NP test, isolates were also subjected to the polymerase chain reaction detection of bla NDM-1 gene. Phylogenetic analysis showed very less variation in bla NDM-1 gene with respect to bla NDM-1 possessing by different bacteria Conclusions: Our findings highlight the incidence of bla NDM-1 in E. coli isolates with a reduced susceptibility to Imipenem or meropenem.

Keywords: NDM-1, carbapenems, M?Ls, Escherichia coli, antibiotics resistance.