European Journal of Biomedical and Pharmaceutical Sciences

PRODUCTION, DETOXIFICATION AND PURIFICATION OF LIPASES USING DE-OILED SEED CAKE AS A SUBSTRATE

Asmita R. Shringeri* and G. R. Naik

ABSTRACT

Lipases (triacylglycerol acylhdrolases, EC 3.1.1.3) catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. Lipases occur widely in nature, but only microbial lipases are commercially significant. Lipases are enzymes that can be secreted by several microorganisms able to produce these enzymes. Many agro-industrial residues can be used as potential substrate for production of enzymes. Lipolytic bacteria was isolated from contaminated garage soil sample and grown on tributyrin media containing 1% of Tributyrin. This article discusses the enzyme assay, production of lipase using solid state fermentation, detoxification of seed cake, purification of lipase using ammonium sulphate precipitation, column chromatography, gel filtration chromatography and use of microbial lipases. Our results suggested that solid state fermentation process is a tool to utilize the low-cost substrate like simarouba seed cake, jatropha seed cake, pongamia seed cake for the production of industrially important enzyme such as lipase. In view of the increasing understanding of lipases and their many applications in high-value syntheses and as bulk enzymes, these enzymes are having an increasing impact on bioprocessing.

Keywords: Lipases, Detoxification, Solid state fermentation, Column Chromatography, Gel Filtration Chromatography SDS-PAGE.