European Journal of Biomedical and Pharmaceutical Sciences

FLOW CYTOMETRY ANALYSIS OF CRUDE SAPONIN FROM THE LEAVES OF MANGIFERA INDICA AND ANTHOCEPHALUS CADAMBA FOR ITS ANTI-INFLAMMATORY ACTIVITY

Amit Gupta and Sushama R Chaphalkar*

ABSTRACT

Crude saponin extracts of Mangifera indica and Anthocephalus cadamba were screened for anti-inflammatory activity using hepatitis vaccine as antigen on human whole blood and animal model studies. For the separation of saponin using thin-layer chromatography was used to identify the type of saponins present in the crude extracts. The immunological studies of the crude saponin extracts gave the following intraperitoneal injection of hepatitis vaccine (20 μg/ml) on day 0 and 7 and extract (10, 30 and 100 mg/ml) continuously given from day 0 to day 9 for the estimation of surface markers (CD3/CD4/CD8) and peritoneal macrophages activation. The anti-inflammatory study of the saponin crude extracts showed statistically significant decrease in the monocyte count as compared to control in human whole blood where as in case of animal model studies, there is decline in surface markers (CD3/CD4/CD8) and peritoneal macrophages activation. The study showed that the anti-inflammatory properties attributable to these plants may be due to their saponins contents.

Keywords: Mangifera Indica, A. Cadamba, Anti-Inflammatory.