EVALUATION OF ANTI-ULCER ACTIVITY OF ETHANOLIC EXTRACT OF SEEDS OF HORDEUM VULGARE IN RATS
L. Srividya and A. Rama Narsimha Reddy*
ABSTRACT
The present study was aimed to evaluate the anti-ulcer activity of ethanolic extract of Hordeum vulgare on ulcer formation and gastric acid release in rats. Pylorus ligation method was used to induce gstric ulcers in rats. Five groups of male wistar albino rats were used in the study. Group 1 serves as normal control group, Group 2 serves as ulcer control, Group 3 treated with 100mg/kg of test compound and Group 4 treated with 200mg/kg of test compound and Group 5 treated with 3.6mg/kg of Omeprazole and serves as standard control group. Before treatment with test and standard drugs ulcer was induced by tight ligation of pylorus portion of stomach. Immediately after pylorus ligation test and standard drugs were administered. After 19 hours rats were sacrificed and ulcer index, percentage protection was determined. The stomach contents were analyzed for gastric acid volume, gastric acid pH and gastric acidity. Both Omeprazole and test compound showed significant reduction (P< 0.01) in ulcer index, gastric acidity and increase in gastric acid pH. Test compound has showed more significant effect (p<0.05) over Omeprazole in decreasing the gastric acid volume. Treatment with test compound showed significant protection against ulcers induced by pylorus ligation. Histological studies revealed the ulcer control group exhibited severe damage of gastric mucosa compared to rats treated with test compound and omeprazole which showed gastric mucosal protection. The present findings suggest that test compound promotes ulcer protection as ascertained grossly and histologically as compared to the ulcer control group. In conclusion test compound possesses anti ulcer property which might be due to its antisecretory activity.
Keywords: Hordeum vulgare, pylorus ligation, Ethanol, antiulcer activity, ulcer index, gastric acid volume.
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