European Journal of Biomedical and Pharmaceutical Sciences

A PHARMACOGNOSTICAL AND PHYTO-CHEMICAL ASSAY OF KANCHANARA GUGGULU – A PROMISING MEDICINE FOR BEEJAKOSHA GRANTHI (OVARIAN CYST)

*Vd. Pooja Vihariya, Dr. Shilpa Donga, Harisha C.R. and Shukla V.J.

ABSTRACT

Introduction: Women have many Diseases in common, Ovarian cysts are at the top of the list. An Ovarian cyst is a fluid-filled sac that forms in the ovary. Ovarian cysts affect fertility if they interfere with normal ovulation or represent a mechanical obstacle for the fertilization process. In Ayurved context to Granthi, situated in Beejakosha can be correlated with ovarian cyst. Kanchanara Guggulu has been selected in present study. Till date there is no scientific evaluation has been observed about the Pharmacognosy and Pharmaceutical study. Aim: Authentication of raw drug of Kanchanara Guggulu and Phyto-chemical evaluation of finished product. Material & Methods: The present study deals with the Pharmacognostical identification of the ingredients of Kanchanara Guggulu and its physicochemical analysis. High-Performance Thin Layer chromatography study (HPTLC) was also developed. Results: Pharmacognostical results shows Black debris of Maricha, Epidermal cells of Patra, Group of stone cells of Maricha & Pippali, Lignified scleroids of Haritaki, Lignified stone cells of Pippal, Oil globule of Patra & Twak, Prismatic crystal of Kanchanara, Rhomboidal crystals of Varuna, Scleroids of Amalaki, Haritaki & Bibhitaki, Silica deposition of Amalaki, Starch grains of Sunthi, Stone cell of Kanchanara & Haritaki, Trichome of Bibhitaki. Qualitative study shows that pH is 6.5, Ash value is 14.1% w/w, Loss on drying is 9.26% w/w, Water soluble extraction is 27.2% w/w & Alcohol soluble extraction is 9.64% w/w, Acid soluble Ash 6.05%w/w, Uniformity of tablet-Maximum weight 3.120gm, Minimum weight 2.80gm, Average weight 2.961gm,Tablet hardness 4.75kg/cm2. High-performance thin layer chromatography (HPTLC) revealed maximum 13 spots in short wave ultraviolet (UV) 254 nm and 12 spots were obtained in long wave UV 366 nm. Conclusion: Pharmacognostical study revealed genuinity of raw drugs. Physicochemical and HPTLC studies inferred that the formulation meets the minimum quality standards. The inference from this study may be used as reference standard in the further quality control researches.

Keywords: Beejakosha Granthi, Kanchanara Guggulu, Pharmacognostical & Phyto-chemical Analysis.