European Journal of Biomedical and Pharmaceutical Sciences

PREVALENCE OF AMPC ?-LACTAMASE GENES AMONG CLINICAL ISOLATES OF ENTEROBACTER SPP. FROM SOME IRAQI HOSPITALS.

Lamiaa M. Jasim and Saad L. Hamed* Department

ABSTRACT

to broad spectrum β-lactams mediated ESBLs and AmpC β-lactamase enzymes is a rising problem worldwide. Determination of their prevalence is essential to the creation of an effective antibiotics policy and hospital infection control measures. Method: A total of 35 clinical isolates of Enterobacter spp. that were collected from different clinical specimens during the period between October 2015 to January 2016 were studied. Antibiogram profile was done by disc diffusion test (DDT), along with recommended tests for phenotypic detection of ESBLs and AmpC production. Isolates were also screened for their possessions of AmpC β-lactamase-encoding genes by using polymerase chain reaction (PCR) technique. Results: Antibiogram revealed that most Enterobacter spp. isolates were multi-drug resistant with a high resistance to cephalosporins; cephalexin(100%), cefazolin and cefotaxime(94.28%), while Susceptibility to imipenem. was highest(20%) followed by amikacin, pipracilli/tazobactam (22.85%).The phenotypic detection of ESBL and AmpC β-lactamases revealed that 25(71.42%), 29(82.85%) were ESBLs and AmpC β-lactamase producers respectively, in addition to 22(62.85%) of isolates were gave positive results for both ESBL and AmpC β-lactamases production. Detection of AmpC encoding genes revealed that a total of 28(96.55%) of 29 Enterobacter spp. were possessed AmpC genes that encoding for AmpC β-lactamases, with predominance of blaCIT, blaEBC and blaDHA genes, 16(57.14%), 11(39.28%), 8(28.57%) respectively. Conclusions: Phenotypic and genotypic methods for detection of AmpC-β-lactamese-producing Enterobacter spp. revealed that most of these isolates were AmpC β-lactamase producers;29(82.85%) and 28(96.55%) respectively, with predominance of CIT 16(45.71%) followed by 10(28.57%) , 8(22.85%) for EBC and DHA respectively. furthermore, 22(62.85%) of isolates were gave positive results for both ESBL and AmpC β-lactamases production. KEYWORDS: Enterobacter

Keywords: Enterobacter spp., Amp C ?-lactamases, ESBLs, PCR.