European Journal of Biomedical and Pharmaceutical Sciences

CYTOPROTECTIVE EFFECT OF LAWSONIA INERMIS LEAVE EXTRACT IN ALUMINIUM- INDUCED OXIDATIVE STRESS ON SEX HORMONES PROFILE IN ADULT MALE WISTAR RAT.

Olawuyi T.S.*, Olotu E.J., Paul C.W., Olorunfemi O.J. and Aina O.S.

ABSTRACT

Henna (Lawsonia inermis) has been used cosmetically and mechanically for over 9,000 years.Metals can cause hormonal imbalance by affecting the neuroendocrine system, disrupting the secretion of androgens from Leydig cells. The aim of this study was to investigate: ‘Cytoprotective effect of Lawsonia inermis Leaves Extract on Aluminium-induced Oxidative stress in the Hormone profile of Adult male Wistar rat’. The objectives were to study the antioxidant effect of Lawsonia inermis leaves extract on aluminum induced oxidative stress on the Hormone profiles: leutenizing hormone (LH), follicular stimulating hormone (FSH) and testosterone. Thirty five adult male Wistar rats were used, with an average weight of 100-196g. Lawsonia inermis extracts and aluminum chloride (AlCl3) were administered for a period of three (3) weeks with five (5) rats per group.Group A (control): Given rat pellets and distilled water. Group B: Given 60mg/kg/d extract of aqeuous Lawsonia inermis. Group C: Given0.5mg/kg/d of AlCl3. Group D: Given 0.5mg/kg/d of AlCl3and low dose 60mg/kg/dof aqueous Lawsonia inermis orally. Group E: Given 0.5mg/kg/d of AlCl3and medium dose 75mg/kg/d of aqeuous Lawsonia inermis orally. Group F: Given 0.5mg/kg/d of AlCl3 and high dose 100mg/kg/d of aqueous Lawsonia inermis orally. Group G: Given 0.5mg/k/d of AlCl3and 5mg/Kg/d Ascorbic acid in distilled water orally. Twenty four hours after the last administration, the animal were weighed and thereafter sacrificed by the use of chloroform as a sedative. The organs were located, removed and weighed using an electronic sensitive analytical balance (Sartorious). Results, showed no significant changes in the weight of Pituitary gland and brain but there was significant changes statistically on the hormone profile. In conclusion, Lawsonia Inermis has been clearly shown to increase reproductive hormones significantly, rendering free radical harmless and markedly lessens stress deleterious effect.

Keywords: Oxidative Stress, Sex hormones, L. inermis.