European Journal of Biomedical and Pharmaceutical Sciences

MOLECULAR DETECTION OF CTX-M-?-LACTAMASES IN PSEUDOMONAS AEROGENOSA STRAINS ISOLATED FROM CORNEAL INFECTIONS

Ali Mhana Sabeeh Zirjawi, Nadheema Hammood Hussein, Buthainah Mohammed Taha* and Jumaah Dakel Hussein

ABSTRACT

Bacteria producing CTX-M- β -lactamases are emerging around the world as a source of resistance to oxyiminocephalosporins such as cefotaxime (CTX). This study was aimed to detect the presence of blaCTX-M gene among Pseudomonas aerogenosa (P. aerogenosa) strains isolated from corneal infections in Ibn- Al haitham teaching hospital for eyes in Baghdad. Eleven P. aerogenosa strains were isolated from specimens of corneal infections of patients at Ibn- Al haitham teaching hospital during four months (April- July, 2016).. The results of antibiotic susceptibility of P. aerogenosa strains showed most strains were resist to most antibiotics under test and the highest resistant antibiotic was Cefazolin which gave 11(100%) resistance rate followed by Ampicillin, Aztreonam, Ampicillin-Sulbactam and Amoxicillin-clavulanic acid which gave 9(81.81%) resistance rate. On the other hand the most effective antibiotic under test was Amikacin which it gave the lowest resistance rate 2(18.18%) followed by Chloramphinical which it gave 4(36.36%) resistance rate. Phenotypic detection of ESBL producing strains was done by screening test and the results showed that out of 11 P. aerogenosa, 7(63.64%) were positive ESBL producing strains while 4(36.36%) P. aerogenosa strains were negative ESBL producing strains. Molecular detection of blaCTX-M showed that out of 11 P. aeruginosa strains, 3(27.27%) were harbored the blaCTX gene (544bp) and those 3 strains were also positive ESBL producing strains by phenotypic detection, while 8(72.72%) strains not harbored blaCTX-M gene.

Keywords: Pseudomonas aerogenosa, Extended-Spectrum ?-Lactamases (ESBL), CTX-M gene and corneal infections.