Abstract
MOLECULAR DETECTION OF CTX-M-?-LACTAMASES IN PSEUDOMONAS AEROGENOSA STRAINS ISOLATED FROM CORNEAL INFECTIONS

Ali Mhana Sabeeh Zirjawi, Nadheema Hammood Hussein, Buthainah Mohammed Taha* and Jumaah Dakel Hussein

ABSTRACT

Bacteria producing CTX-M- β -lactamases are emerging around the world as a source of resistance to oxyiminocephalosporins such as cefotaxime (CTX). This study was aimed to detect the presence of blaCTX-M gene among Pseudomonas aerogenosa (P. aerogenosa) strains isolated from corneal infections in Ibn- Al haitham teaching hospital for eyes in Baghdad. Eleven P. aerogenosa strains were isolated from specimens of corneal infections of patients at Ibn- Al haitham teaching hospital during four months (April- July, 2016).. The results of antibiotic susceptibility of P. aerogenosa strains showed most strains were resist to most antibiotics under test and the highest resistant antibiotic was Cefazolin which gave 11(100%) resistance rate followed by Ampicillin, Aztreonam, Ampicillin-Sulbactam and Amoxicillin-clavulanic acid which gave 9(81.81%) resistance rate. On the other hand the most effective antibiotic under test was Amikacin which it gave the lowest resistance rate 2(18.18%) followed by Chloramphinical which it gave 4(36.36%) resistance rate. Phenotypic detection of ESBL producing strains was done by screening test and the results showed that out of 11 P. aerogenosa, 7(63.64%) were positive ESBL producing strains while 4(36.36%) P. aerogenosa strains were negative ESBL producing strains. Molecular detection of blaCTX-M showed that out of 11 P. aeruginosa strains, 3(27.27%) were harbored the blaCTX gene (544bp) and those 3 strains were also positive ESBL producing strains by phenotypic detection, while 8(72.72%) strains not harbored blaCTX-M gene.

Keywords: Pseudomonas aerogenosa, Extended-Spectrum ?-Lactamases (ESBL), CTX-M gene and corneal infections.


[Full Text Article]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: FEBRUARY ISSUE PUBLISHED

    FEBRUARY 2023 Issue has been successfully launched on 1 FEBRUARY 2023.

  • EJBPS New Impact Factor

    Its our Pleasure to Inform you that EJBPS Impact Factor has been increased from 5.467 to 6.044, due to high quality Publication at International Level.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT