European Journal of Biomedical and Pharmaceutical Sciences

THE EFFECT OF K3EDTA AND K2EDTA ANTICOAGULANTS ON COMPLETE BLOOD COUNT

Mohammed Osman Mahmoud* and Enaam Abdelrahman Abdelgader*

ABSTRACT

Background: Complete blood counts are done to monitor overall health, to screen for some diseases, to confirm a diagnosis of some medical conditions, to monitor a medical condition, and to monitor changes in the body caused by medical treatments. In laboratory medicine, pre- analytical quality control is very important, Blood-drawing tubes made from plastic containing K2EDTA as an anticoagulant are gaining widespread use in clinical hematology. Ethylene diamine tetra acetic acid (EDTA), is a poly amino carboxylic acid and a colourless, water-soluble solid. Its conjugate base is named ethylene diamine tetra acetate. It is widely used to dissolve timescale. Its usefulness arises because of its role as a hexadentate ("six-toothed") ligand and chelating agent, i.e. its ability to "sequester" metal ions. Objective: This study aimed to assess the effect of both K3EDTA glass tubes and Vacutainer K2EDTA plastic tubes on fresh and after six hours on Complete Blood Count Parameters. Materials and Methods: This was a comparative cross-sectional study conducted in Khartoum state, Sudan in 2016. It included 100 samples and 400 tests have done, The CBC was done with plastic tubes Vacutainer K2EDTA on fresh sample and after 6 hours and glass tubes Vacutainer K3EDTA on fresh sample and after 6 hours, Sysmex® Kx-21N hematological analyzer used to estimate CBC. Results: There was a significant difference in HCT, MCHC, MXD%, MXD#, RDW_sd, PDW, and P-LCR, with P.values (.001),(.006) (.049), (.019),(.018) and (.033) respectively. Conclusion: This study concluded that effect of K3EDTA and K2EDTA on Complete Blood Count Parameters caused significant difference in HCT, MCHC, MXD%, MXD#, RDW_sd, PDW, and P- LCR, (p-value < 0.05).

Keywords: Complete blood count, K3EDTA, K2EDTA.