European Journal of Biomedical and Pharmaceutical Sciences

ANTIBACTERIAL AND PHYTOCHEMICAL INVESTIGATION OF DATURA STRAMONIUM L LEAF EXTRACTS AGAINST CLINICAL WOUND SAMPLES

A. Ananth and S. Rajan*

ABSTRACT

Context: Datura stramonium Linn is one of the most common Indian medicinal plants commonly Used as medicine to cure various diseases. This study determined the presence of virulence factors in the organism, correlates it with the multi-drug resistance (MDR) and antibacterial, phytochemical activities of the leaf of Datura stramonium against wound causing pathogens. Aims: The aim of the following study is evaluate the antibacterial, phytochemical activity of the different extracts of D. stramonium leaf sample against selected bacterial isolates. Subjects and Methods: Purulent materials from different wound infections samples received were processed as per standard microbiological procedures. Virulence factors of biofilm were studied. Antibiotic sensitivity test was performed by standard Kirby Bauer disc diffusion technique. Antimicrobial activity of various extracts of plant leaves was investigated by agar disc and well-diffusion method against wound causing bacterial isolates. The phytochemical activities of leaf extract were done as per Clinical and Laboratory Standard Institute Guidelines. Results: Each collected wound samples showed one or more bacterial isolate, totally 78.9% of samples exhibited 43 isolates. Among the 18 antibacterial agents, the most of the antibacterial agents had highly resistance activity and some agents had highly sensitive activity against the isolates. All isolates were having the ability to produce biofilm, among them 58% of isolates were strong Biofilm producers. The antibacterial studies, the acetone extract of Datura stramonium presented the highest anti-S. aureus activity and was effective against all bacterial strains tested. Phytochemical screening of the plant revealed the presence of alkaloids, tripenoid, steroids, flavonoid, triterpenes, phenolic compounds and tannins. Conclusions: The general results observed consider the plant as natural source of phytoconstitution quality for antimicrobial effectiveness.

Keywords: Wound, Clinical bacteria, extracts, antibacterial, photochemical, Datura stramonium.