European Journal of Biomedical and Pharmaceutical Sciences

QUANTITATIVE ESTIMATION OF PROTEIN USING NATURAL DYES EXTRACTED FROM CAESALPINIA PULCHERIMA PLANT FLOWERS

Bharati K. T.*, Gujarathi D. B., Pardhi T. R., Varpe R. B. and Deshmukh S.S.

ABSTRACT

A new photometric method for the quantification of protein using the dye extracted from Purple, Yellow and Red color flowers of Caesalpinia pulcherima plant has been developed In the present study, we have used aqueous, acid, alkali and methanolic extract of above said flowers. The extracted dyes were used in the protein-dye binding studies. Out of that the methanolic extract show the stable color of protein-dye complex with increase in concentration of protein. The methanolic extract for three dyes achieved linearity up to the following concentration of protein, Purple color dye (80μg); Yellow color dye (90 μg) and Red color dye (80 μg).

Keywords: Caesalpinia pulcherima, Natural dye and protein-dye complex.