ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE T-786C POLYMORPHISM AMONG TYPE-2 DIABETES MELLITUS PATIENTS IN KHARTOUM, 2016
Sara Mudawi and Ibrahim Khidir Ibrahim*
ABSTRACT
Background: A single nucleotide polymorphism (T > C) rs2070744 due to transition of a thymine to a cytosine at T-786C in the promoter region of eNOS was found to reduce the rate of mRNA transcription by 50%, resulting in decreased serum NO levels which can inhibit apoptosis or stimulate tumor proliferation, angiogenesis and metastasis. Objective: To evaluate the association between Nitric Oxide Synthase gene T-786C polymorphism and type-2 Diabetes mellitus patients in Khartoum. Material and Methods: A total of 40 patients with type-2 Diabetes mellitus and50 control subjects were enrolled this study. DAN extrication samples previously extracted from EDTA Blood collected from SPV and store at- 20C. Analysis of the eNOS786T > C promoter polymorphism were carried out by allele specific polymerase chain reaction method (PCR). Results: The present study reported that, molecular analysis showed that, the most frequent genotype in patients was TT 100% (40 sample). While in the control group there was 100% TT genotype. Conclusion: The present study indicates the lack of association between eNOS and type-2 Diabetes mellitus which needs to be investigated in study with larger sample.
Keywords: eNOS and type-2 Diabetes mellitus.
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