European Journal of Biomedical and Pharmaceutical Sciences

A REVIEW ON ESTIMATION OF LOCAL ANAESTHETICS BY HIGH PERFORMANCE LIQUID CHORMATOGRAPHICMETHODS

D. Lavanya*, J. Neeharika, D. Teja, P.V. Suresh and P. Sai Geervani

ABSTRACT

The main goal of this review is to provide overview on the Reverse phase high performance liquid chromatography and normal phase high performance liquid chromatographic methods that are necessary to analyse the local anaesthetics. Local anaesthetics are used in a wide range of clinical situations to prevent acute pain and to stop or ameliorate pain produced by cancer or pain associated with chronic painful conditions. These have similar chemical structure but differing pharmacokinetic properties and spectra of pharmacodynamics effects that influence selection of agents for use in various clinical situations. Local anaesthetics (LAs) are drugs which can applied upon topical application or local injection cause reversible loss of sensory perception, especially of pain, in a restricted area of the body. These classes of drugs are mainly analysed by using Reverse phase HPLC in which stationary phase non-polar and mobile phase is polar and Normal phase HPLC in which stationary phase is polar and mobile phase is non-polar.

Keywords: Reverse Phase –Hplc, Hplc, Lidocaine, Bupivacaine, Local Anasthetics.