European Journal of Biomedical and Pharmaceutical Sciences

PRODUCTION AND CHARACTERIZATION OF ENDOGLUCANASE FROM BACILLUS SUBSTILLS BY USING WHEAT BRAN AS SUBSTRATE

S. Nasireen Begum* and S. Rizwana Begum

ABSTRACT

Microbial cellulases have great significance due to their wide range of applications from industrial sector to household products and comprise 60% of worldwide enzyme market. cellulase are used for a variety of industrial applications, such as commerical food processing in coffee, cellulases are widely used in textile industry and laundry industry, pulp and paper industry, Pharmaceutical industryalso used in fermantation of biomass. Ideally, Endoglucanase used in industries should have good activity over a wide range of pH and temperatures .Because, most of the industrial practices are carried out at higher temperature and broad range of pH most of the enzymes are unstable. Therefore, there is a great demand and scope for screening thermostable and alkaliphilic endoglucanase that can tolerate the adverse industrial condition This has attracted the attention of many researchers throughout the world for screening of potential Endoglucanase from various microbial strains viz., Aspergillus sp., Bacillus sp., Clostridium sp., Mucor sp., Pseudomonas sp., etc. These microbes were successful in producing endoglucanase and their yields can also be enhanced by optimizing the media components and various physical parameters such as pH, temperature and agitation or both. It was also found that simple sugars have catabolic repression whereas complex carbon and nitrogen substrate like casein, chicken feather, shrimp shell powder etc., might act as inducers for endoglucanase production. Therefore the current study is focused on Production and characterization of endoglucanase from Bacillus substills by using wheat bran as substrate.

Keywords: Cellulase, Endoglucanase, Wheat Bran, Bacilus Substils.