European Journal of Biomedical and Pharmaceutical Sciences

METHANOLIC EXTRACTS: ANTI-ATHEROTHROMBOSIS ACTIVITIES

Md.Torequl Islam*, Ayesha Mahmood, Abdullah Al Faruq and Ali Ahmmad

ABSTRACT

Thrombus formation inside the blood vessels obstructs the blood flow through the circulatory system leading to hypertension, heart stroke, anoxia and so on. The complete deprivation of oxygen during infarction is the cause of the cell death. Crude biologicals and their components possessing antithrombotic activity have been reported before. This study was aimed to investigate thrombolytic activity of methanol extracts of four traditionally used medicinal plants. For this an in-vitro thrombolytic study was carried out along with streptokinase and methanol were taken as reference standard and negative control respectively. Two doses (5 μg/μl and 10 μg/μl) were selected for the methanolic crude extractives. The highest lysis (55.15%) activity was observed with the Plumbago indica extract at the dose of 10 μg/μl. Then followed by 28.29%, 26.53% and 17.26% for the Ficus racemosa, Alstonia macrophylla and Alstonia scholaris respectively at the same dose. With some exceptions all the extractives showed graded dose responses. Preliminary chemical group identification test revealed the presence of alkaloids, glycosides, steroids, saponins and terpenoids, tannins and reducing sugars important secondary metabolites.

Keywords: Extracts, methanol, streptokinase, thrombolytic activity.