THE EFFECT OF PLANT GROWTH HORMONES (AUXINS AND CYTOKININS) ON IN-VITRO SHOOTING AND ROOTING ABILITY OF POTATO NODAL CULTURE
Yalemtesfa Firew Guade*
ABSTRACT
An experiment was carried out in Jimma University department of horticulture tissue culture laboratory using single nodal cuttings (stem segments having one auxiliary bud) taken from a released potato variety Gudane as an explant. The experiment was conducted using complete randomized design (CRD) having four replications with the objective of developing the optimum level of combination of auxin to cytokinin ratio for regeneration of potato plant from a single nodal culture and identifying the effects of different levels of hormonal combination on shooting and rooting of ability of potato nodes cultured in vitro. The nodal cuttings were cultured on Murashige and Skoog basal media supplemented with different levels of concentrations of growth hormones of Naphthalene Acetic Acid (NAA) and Benzyl Adenine (BA) with combinations of [(0, 4), (3, 2) , (2, 3) and (4, 0) mg/l)] keeping all other components of MS basal media to be constant. Data were collected on number of nodes per plant, number of shoots per plant and number of roots per plant. The results of statistical analysis showed treatment combination having 4mg/l of BA without NAA significantly increased the number of shoots and nodes formed per plant. The largest number of roots per plant was obtained from T3, T2 and T4 with hormonal combinations of (4, 0), (3, 2) and (2, 3), respectively. T5 assigned as control without any hormonal combination indicated the least performance in number of shoots and roots per plant. Therefore, T2 with hormone combination of 3mg/l NAA and 2mg/l BA is identified as the optimum level of combination of growth hormones for shooting and rooting of potato nodal culture in-vitro condition.
Keywords: Nodal cutting, explant, optimum protocol, MS basal medium.
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