European Journal of Biomedical and Pharmaceutical Sciences

PRODUCTION, CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY OF A BIOEMULSIFIER PRODUCED BY S.CEREVISIAE UTILIZING EDIBLE OILS

*Naveen Banu M. and Dr. Harikrishnaraj R.

ABSTRACT

This study was designed to evaluate the ability of bioemulsifier to inhibit the growth of some pathogenic microorganisms. Commercially available Saccharomyces cerevisiae were tested for their ability to produce bioemulsifier. The production of bioemulsifier was detected by determination of emulsification index (E24%), qualitative drop-collapse test, emulsification activity (E.A) and Oil displacement. The results of bioemulsifier produced by Saccharomyces cerevisiae cultivated in Cooper and Paddock’s broth containing different types of oils were: E24%= 66.6%, E.A= 184EU/ML and 1.8mm of oil displacement . Bioemulsifier extraction and solubilization was done by boiling (100º C) S. cerevisiae cells for 30 min in water, 0.067M phosphate buffer (PB) and 0.067M PB with 0.02M Na2S2O5. The antimicrobial activity of extracted bioemulsifier was tested against some pathogenic microorganisms. Results revealed that bioemulsifier reduced the growth of bacteria (Escherchia coli and Staphylococcus aureus).

Keywords: Bioemulsifier, Emulisification activity, Emulsification Index, Antimicrobial Activity.