METHANOL PLANT EXTRACT OF RUMPUT TEKI (CYPERUS ROTUNDUS L.) CAUSING FETAL SKELETON RETARDMENT IN MICE
Nuning Nurcahyani, Yan Wirasti, Jamsari, Djong Hon Tjong and Mohammad Kanedi*
ABSTRACT
Rumput teki (Cyperus rotundus L.) is a weed plant possesses biological and pharmacological activities and commonly used in treatment of various disease in many folk remedy systems. However, scientific studies on the safety and biological side effects of this plant use is still limited. This study aimed to investigate the possible effects of crude extract of nut grass (Cyperus rotundus L.) on the skeletal development of mice (Mus musculus L) during organogenesis period. Pregnant females (n=24) divided into four groups consisted of six mice each. Group 1 treated orally with distilled water (as control), whereas group 2, 3 and 4 were consecutively given extract at the dose of 45 mg/40g BW, 90 mg/40g BW and 135 mg/40g BW. After being treated for 12 days (day 6 to day 17), on the day 18 the fetuses were taken by caecarean section. The fetus count, fetal weight and length, and fetal tibial epiphyseal cartilage zones thickness namely the zone of reserve, proliferation, maturation, and calcification were assessed as the study parameters. The results showed the number, weight and length of fetus, the thickness of reserve, proliferation and maturation zones significantly lower by the higher dose of extract. In contrast, the thickness of calcified cartilage zone was increased by the increase of the dosage. Thus, it can be concluded that methanolic plant extract of rumput teki causing fetal skeleton retardation in mice.
Keywords: Teki, nut grass, Cyperus rotundus, epiphyseal cartilage, fetal retardation, chondrocyte, calcification.
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