European Journal of Biomedical and Pharmaceutical Sciences

PHARMACOGNOSTIC AND ANTIMICROBIAL SCREENING STUDIES OF EUGENIA JAMBOLANA SEEDS

Harinderjit Singh and Dr. Hayat M. Mukhtar*

ABSTRACT

Eugenia jambolana is a large evergreen tree indigenous to the Indian subcontinent belonging to the family Myrtaceae. Plants are rich in a variety of secondary metabolites such as tannins, terpenoids, alkaloids, flavonoids, phenols, steroids, glycosides and volatile oils that serve as natural plant defence mechanisms against invasion by microorganisms, insects, and herbivores or for combating infectious or parasitic agents or generated in response to stress conditions (Cowan, 1999). Eugenia jambolana have promising therapeutic value with its various phytoconstituents such as tannins, alkaloids, steroids, flavanoids, terpenoids, fatty acids, phenols, minerals, carbohydrate and vitamins. In a study, the antimicrobial activity of E. jambolana leaves extract was done against clinical isolates by using well diffusion method. The different extracts showed inhibitory activity against clinical isolates of gram negative bacteria such as Salmonella typhi , Shigella dysentriae, Kelbisiella pneumonia, Pseudomonas aeruginosa, Ecoli, and gram positive bacteria such as Bacillus subtilis, Staphylococcus aureus (Jetthu et al., 2011; Parkash et al., 2011). Antimicrobial activity using (Agar well diffusion assay). Amongst various extracts (EJPE, EJC, EJM and EJAQ) of E. jambolana tested, methanol extract (EJM) exhibit significant antimicrobial activity. Finally, bioactive extract was subjected to the chromatographic separation of chemical compound which was further characterized by using spectroscopic method and the isolated compound was found to be 3-(2,2,3,3,4,5,5,6,6-nonamethylcyclohexyloxy)-3,4-dihydro-2.

Keywords: Eugenia jambolana seeds, Pharmacognostic, Antimicrobial study, Medicinal plants.