European Journal of Biomedical and Pharmaceutical Sciences

PREPARATION AND EVALUATION OF GLIBENCLAMIDE- ALGINATE MICROSPHERES

*Y. Dastagiri Reddy, V. Ravisankar, P.Ravi Prakash, K.Shravani, P.Arun Kumar, A.B.Shravani

ABSTRACT

Glibenclamide is an oral hypoglycemic agent used in the treatment of non-insulin dependent diabetes. It is a weak acid and is poorly soluble in water. In the present study microspheres were prepared by ionic cross-linking technique. Chemical reaction between sodium alginate and calcium chloride to form calcium alginate was utilized for microspheres. For slowing the rate of release from microspheres Eudragit RS100 and Xanthan gum combinations were added, so that the drug will be release constantly for 12hrs. The prepared microspheres were characterized in terms of drug-excipient compatibility study, percentage yield, micromeritics study, swelling index, particle size analysis, shape analysis, drug content, drug encapsulation efficiency and in-vitro drug release study. To analyze the mechanism of drug release from the tablets, the In-vitro dissolution data of optimized formulations were fitted to zero order, first order, higuchi release model and korsmeyer-peppas model based on regression coefficient. The n values of the optimized formulations F-8 and F-7 were 0.572 and 0.548 respectively. This indicates the release of drug followed Non Fickian or anomalous transport. Results signified the fact that, microspheres formed has sufficient good surface and size to be utilized as a dosage form responsible for slow release of drug from matrix through erosion. Among all the nine batches formed. F8 was selected as the optimized batch in terms of all parameters evaluated.

Keywords: Glibenclamide, Ionic cross-linking technique, Eudragit RS100, Xanthan gum.