European Journal of Biomedical and Pharmaceutical Sciences

STABILITY INDICATING RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF TRANEXAMIC ACID AND MEFENAMIC ACID IN TABLET DOSAGE FORM

Priyanka R. Tiwari*, Dr. Anuradha G. Patel, Shailesh V. Luhar and Dr. Sachin B. Narkhede

ABSTRACT

To develop simple, accurate, precise, rapid and economical Stability Indicating RP-HPLC method for the Tranexamic acid and Mefenamic acid in dosage form method included Shimadzu LC-2010, using Hypersil BDS- C18 (250 * 4.6 mm, 5μm) column and with mobile phase composition of Phosphate Buffer: Acetonitrile (70:30 % v/v)pH 6, at a flow rate of 1ml/min was used. Detection was carried out at 215 nm. Retention time of Tranexamic acid and Mefenamic acid was found to be 5.277 min and 3.583 min. For Stability Study Drug Were Subjected to acid hydrolysis, alkaline hydrolysis, oxidative degradation and thermal degradation. The linear of the Proposed method was investigated in the range of 10-30 μg/ml and 5-15 μg/ml for Tranexamic acid and Mefenamic acid. The limit of detection were0.704 μg/ml and 0.908 μg/ml of Tranexamic acid and Mefenamic acid and the limit of quantification were 2.156 μg/ml and 2.754 μg/ml of Tranexamic acid and Mefenamic acid.

Keywords: Tranexamic acid, Mefenamic acid RP-HPLC method, forced degradation, validation.