European Journal of Biomedical and Pharmaceutical Sciences

IN VITRO ANTICANCER ACTIVITY OF ENDOPHYTIC FUNGAL EXTRACTS FROM CLADOSPORIUM FAMILY ISOLATED FROM CYATHOCLINE PURPUREA (D. DON.) O. KTZE

Harsha D. Pednekar, Dr. Harsha V. Hegde*, Pramod J. Hurakadale, Kishore Bhat, Chetana Bogar and Jyoti Wagawade

ABSTRACT

Cyathocline purpurea (D. Don.) O. Ktze. (Asteraceae) is a well documented anticancer plant, demonstrating activity against breast cancer cell line- MCF-7. In our present investigation, comparison of cytotoxic potential of 13 extracts from 5 endophytic Cladosporium species namely Cladosporium sphaerospermum Penz, Cladosporium aff. cladosporioides (Fresen.) de Vries, Cladosporium cladosporioides (Fresen.) de Vries and Cladosporium oxysporum Berk. and M. A. Curtis. isolated from leaves of C. purpurea were studied. Axenic cultures of Cladosporium species were isolated from C. purpurea leaves following surface sterilization of leaves. Radial growth in agar plate was compared using different media viz. SDA, SDMA, CDA, YESA and PDA and accordingly SDA/SDB was selected. Culture were grown in SDB media and extracts were prepared using different solvents viz. EA, CHL, HD and HA extract to ascertain cytotoxicity against MCF-7 breast cancer cell line. Most of the Cladosporium fungal extracts demonstrated good cytotoxicity against MCF-7 estrogen positive breast cancer cell line. Interestingly, Cladosporium cladosporioides (Fresen.) de Vries which shares affinity with Cladosporium aff. cladosporioides (Fresen.) de Vries at the species level, fared better in MTT cytotoxic profile with respect to MCF-7 breast cancer cell line. Further analysis of these 2 strains may shed light on correlation of their genetic profile with respect to their cytotoxic potential. Our results indicated that use of Cladopsporium extract can be a good secondary source for possible antineoplastic agent. However, further work is required for screening their potency against various cell lines, both in cancer and in normal.

Keywords: Cyathocline purpurea; MTT; Cladosporium; cytotoxicity; MCF-7; media optimization.