ENHANCED IN VITRO CYTOTOXICITY EFFECT OF GALLIC ACID IN MCF7 CELLLINES BY USING CHITOSAN NANOPARTICLES AS A TARGET VECTOR
Sandhiya V.*, Ubaidulla U. and Priyanka Sinha
ABSTRACT
Cancer is the second leading cause of death worldwide. There is always a huge demand for novel anticancer drugs and scientists explore various natural and artificial compounds to overcome this. Gallic acid (GA) is one of the phenolic acids found in many dietary substances and herbs used in ancient medicine. It possesses antiinflammatory, antioxidant, antiviral and antibacterial properties. The main objective of this work was to prepare and characterize chitosan loaded gallic acid nanoparticles. The organoleptic propertity of the drug were complys with USP standard. The solubility studies were performed. The FTIR studies were carried out for pure drug, polymer and drug with polymer in different ratio which shows there was no chemical interaction between the drug and polymer. The drug content and entrapment efficiency of the nanoparticle was increase as the concentration of polymer increase. The F4 formulation shows more entrapment efficacy compared to other formulations nearly 70.87% and the drug content was found to be 86.7%. The particle size and the surface morphology results revealed that gallic acid nanoparticles (GNPs) were smoothed spheroidal with a size ranging from100-200nm. The invitro dissolution studies were carried out for all the Formulation. Formulation F4 shows 97.67% of drug release at the end of 24 hr. in this study we extensively investigated the anticancer effect of GA in human breast carcinoma MCF-7 cells. the result revealed that GA induce apoptosis by triggering the extrinsic or Fas/ FasL pathways.
Keywords: Gallic acid, MTT- assay, MCF-7 cell line, nanoparticles.
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