European Journal of Biomedical and Pharmaceutical Sciences

DIVISION OF ALLELES IN THE DETERMINATION OF KINSHIP IN SMALL LOCAL POPULATIONS

Jasmin Mušanovi?*, Azra Metovi?, Esad Pepi?, Belma Imamovi?, Amela Džubur- Ali?, Marijana Filipovska- Mušanovi? and Damir Marjanovi?

ABSTRACT

In this study the efficacy of short tandem repetitive sequences - STR was examined to prove kinship among the descendants of one parent pairs within the small local population by analyzing sharing of alleles per locus at siblings and non-siblings of the two villages: Vukotići and Orahovica and comparing these results with the results of a heterogeneous Bosnian-Herzegovinian population of our previous research. There was a significant difference in this division in the group of siblings and a group of non-siblings as well and the phenomenon of polarization when it comes to the representation of sharing zero and two alleles in the samples for both villages, with significantly overrepresented sharing of zero alleles at non-siblings and significantly overrepresented sharing of two alleles at siblings. By comparing sharing of alleles between sample from the small local population and the sample from mixed population from our earlier research, there is no statistically significant difference in the distribution of 2, 1 and 0 alleles between samples of these two population. This obtained ratio of dividing 2, 1 and 0 alleles in these two samples is about that both samples belongs to the same Bosnian-Herzegovinian population. Number of loci that differ from the Hardy-Weinberg equilibrium is different in the village of Orahovica and in the village of Vukotići. One of the possible reasons is the fact that Vukotići is less than the Orahovica. Using STR system in determining the kinship, or lack of the kinship in a small local populations within the Bosnian-Herzegovinian population, is proved to be a successful method based on the results of this research.

Keywords: short tandem repetitive sequences, siblings, non-siblings, allele sharing.