BACTERIAL VAGINOSIS IN REPRODUCTIVE AGE GROUP- A STUDY USING NUGENT SCORING AND WET MOUNT METHODS
R. K. Jha*, M. Dias, M. Vijayasimha, R. P. Jayaswal and Praveen N. M.
ABSTRACT
Introduction: Bacterial vaginosis (BV) is a polymicrobial infection which occurs due to disbalance in normal vaginal flora (Lactobacilli) and overgrowth of Gram negative bacilli specially Gardenella, Prevotella and Mobiluncus. In order to diagnose BV, Nugent's score and Amsel’s criteria has been used as a conventional method with higher reproducibility. Based on Nugent’s scoring, the present study further clarified and highlighted the incidence of significant cases of BV infections in the reproductive age group mainly between 20-30 years. Methods: In present study, vaginal samples were collected from 100 women attending gynecological department having white discharge. The samples were examined for the presence of L. morphotype, G. morphotypes, Prevotella and Mobiluncus by Gram’s staining and analyzed through NS criteria for diagnosis and occurrence of BV. Result: Our study revealed 34% BV positive, 44% BV intermediate and 22% normal cases. Further study had shown co-infection with T. vaginalis and Candida species in BV patients comprising percentage of 3% and 11% respectively. Conclusion: NS is a convenient and inexpensive method to diagnose bacterial vaginosis. L. morphotype, G. morphotypes, Prevotella and Mobiluncus were examined in patient samples in order to diagnose BV. Infection with Candida species were also seen randomly in BV positive and BV negative patient, whereas infections with T. vaginalis only seen in BV positive patients. Our study supported the prevalence of BV in women under reproductive age group with white discharge.
Keywords: Bacterial Vaginosis; White discharge; Gram’s staining; Nugent’s score; L. morphotype; G. morphotypes; Prevotella; Mobiluncus; T. Vaginalis; Candida.
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