European Journal of Biomedical and Pharmaceutical Sciences

ANTIHYPERGLYCEMIC PROTEIN LINEAGE FROM INDIAN MEDICINAL PLANTS

*Dr. Sarika Gupta and Mohini Mendiratta

ABSTRACT

Background: Diabetes is a chronic endocrine disorder caused by lack of insulin and/or reduced insulin activity leads hyperglycemia and abnormalities in carbohydrate, fat-protein metabolism. Numerous medicinal plants have been used for the management of diabetes mellitus in various traditional systems of medicine worldwide as they are a great source of biological constituents and many of them are known to be effective against diabetes due to their antihyperglycemic activities. Methods: This current research work was focused on antihyperglycemic proteins profiling from seeds and leaves of aqueous and methanolic extracts of Momordica charantia, Trigonella foenum-graneum, Triticum aestivum, Solanum tuberosum and Nicotiana tobaccum. Present study was undertaken to analyze the protein profiles from aqueous and methanolic extracts of seeds and leaves of some medicinally important test plants. Crude protein, Osmotin I and II and Polypeptide-P proteins from seeds and leaf. Result: The study indicated the presence of antihyperglycemic proteins, thus supporting its traditional medicinal practices. SDS-PAGE revealed that the band of osmotin I at 26KDa. Osmotin I and osmotin II was recovered from seeds and leaves of aqueous and methanolic fraction of wheat, tobacco and potato. The band of Polypeptide-P was obtained at 11KDa from the aqueous and methanolic extracts of seed as well as leaves of bittermelon and fenugreek. Conclusion: The methanolic extract of test plants seeds and leaves contained antihyperglycemic proteins except polypeptide-p in aqueous fraction. These proteins act as artificial insulin or analogue for insulin/ animal protein adeponectin or thaumatin. These proteins should be subjected to further purification so as to explore the analysis of the mechanism of action under in vivo system i.e.; in animal cell lines to observe the bio efficacy of these components in animal model system. Therefore, it may further be serves as an alternative drug of choice.

Keywords: Antihyperglycemic, Protein lineage, Characterization, Osmotin and Polypeptide P.