CYTOPROTECTIVE ROLE OF HELICTERES ISORA IN CHROMOSOMAL ABERRATIONS AND MICRONUCLEUS FREQUENCIES IN RAT BONE MARROW CELLS AGAINST CISPLATIN INDUCED GENOTOXICITY
Sunny Sharma*, Seema Bhargava and Anil Mehta
ABSTRACT
In the present investigations, the preventive effect of Helicteres isora fruit extract was evaluated against cisplatin induced chromosomal abbreviations and micronucleus frequencies in the rat bone marrow cells. The single PO administration of Helicteres isora methanolic extract at the dose of 200 and 400mg/kg body weight, 24 hours prior the administration of cisplatin (at the dose of 6 mg/kg) significantly prevented the micronucleus formations and chromosomal abbreviations in a dose dependent manner in bone marrow cells of rat as compared to cisplatin group. The highest number of frequencies was observed in rat treated with only cisplatin and least micronucleus frequencies and chromosomal aberrations were observed in 400mg/kg dose group of Helicopters isora (methanolic fruit extract) extract. The animals treated with different doses of Helicteres isora extract, i.e. 200 and 400 mg/kg showed a significant reduction (p<0.05) in the frequencies of micronucleus as well as chromosomal aberrations as compared to cisplatin group. The frequencies decrease with an increase in the dose of extract. There was a significant dose-effect relationship between chromosomal aberrations, micronucleus frequencies and dose of Helicteres isora methanolic fruit extract. It seems to have a preventive potential against Cisplatin-induced mutagenic effect in rat bone marrow cells.
Keywords: Helicteres isora, Mutagenicity, Micronucleus, Bone marrow, Cisplatin, chromosomal abbreviation.
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