COMPARATIVE STUDIES ON PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITIES OF SPINACIA OLERACEA L. AND BASELLA RUBRA L.
Aiyasamy Nishanthini and *G. Umamaheswari
ABSTRACT
Objective: The present study aimed to compare the phytochemical and antimicrobial potential of two Indian spinaches Spinacia oleracea L. and Basella rubra L. Methods: Methanolic extract of Spinacia oleracea and Basella rubra leaves were obtained using Soxhlet extraction techniques. The present study was investigated for invitro antimicrobial activity against selective pathogens using agar well diffusion method. In parallel study was performed to identify the distribution and the concentration of the phytocompounds in the leaves of both plant extracts. Results: The methanolic extract yields were obtained both the spinach as S. oleracea (2.26 g (4.52%)) and B. rubra (2.14 g (4.28%)). The total phenol was estimated in S. oleracea and B. rubra in methanolic extract as 684.5 mg/g of dry extract and 574.2 mg/g dry extract respectively. S. oleracea is found to be better antibacterial activity than the B. rubra. Anticandidal activity was observed in both extract but no activity was observed in A. niger. Both the extracts have all the tested phytoconstitutes except coumorins and glycosides. Conclusion: This comparison study clearly indicated that, the antimicrobial and phytochemical importance of both Indian spinaches. However, the spinach S. oleracea shown higher antimicrobial activity than B. rubra and there is little variation in the phytochemical constituents.
Keywords: Indian spinach, Spinacia oleracea, Basella rubra, Phytochemistry, Antimicrobial activity.
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