CYTOTOXICITY SCREENING OF METHANOLIC BULB EXTRACT OF BELLICORYNE PLUMBAGINIFOLIA AGAINST VARIOUS MAMMALIAN CANCER CELL LINES
*V. Ramamurthy and K. L. Naveen
ABSTRACT
Cancer is a major public health burden in both developed and developing countries. Anticancer activity is the effect of natural and synthetic or biological and chemical agents to reverse, suppress or prevent carcinogenic progression. Several synthetic agents are used to cure the disease but they have their toxicity and hence the research is going on to investigate the plant derived chemotherapeutic agents. Therefore an attempt has been made to review different in vitro method for estimating anticancer properties of natural products from medicinal plants. In this study the anticancer potentials of plants was investigated against Dalton’s lymphoma (DL), MCF-7 and HeLa cells in vitro. Cytotoxicity of various plant extracts was determined by MTT assay. The results showed that the methanolic bulb extract of Bellicoryne plumbaginifolia possessed a moderate amount of anticancer activity and the IC50 value was recorded. The most potent anticancer activity was observed with the methanolic bulb extract of Bellicoryne plumbaginifolia with IC50 values of 51.2μg/ml and 53.1μg/ml and 48.7μg/ml on DL, MCF-7 and HeLa cells respectively. Phytochemical analyses revealed the presence of large amount of alkaloids and flavonoids in the potent plant extracts which may be suggested to play an important role in their anticancer activities. The methanolic extract would be studied further for isolation and characterization of active components for lead optimization studies.
Keywords: Anticancer, cytotoxicity, DL, MCF-7 and HeLa cells, MTT assay.
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