APPLICATION AND ANALYSIS OF THE FOLIN CIOCALTEU METHOD FOR THE DETERMINATION OF THE TOTAL PHENOLIC CONTENT FROM EXTRACTS OF TERMINALIA BELLERICA.
Anjoo Kamboj*, Ritika Gupta, Ankita Rana and Ripanjot Kaur
ABSTRACT
Terminalia bellirica, is a perennial herb mainly distributed in the tropical regions and commonly found in South-East Asia, including Thailand. It is one of the ingredients of “triphala”, an Ayurvedic formulation that is believed to promote health, immunity and longevity. The fruit is reported to have purgative, cardiac depressant,
hypotensive, digestive, expectorant, narcotic, ophthalmic, antipyretic, antiemetic and rejuvenating effect. Unripe fruit is a mild laxative and ripe fruit is an astringent. Seeds are used as aphrodisiac. Oil extract from the seed pulp is used in leucoderma and alopecia. The aim of the present study was to develop and validate an analytical method to determine the content of total polyphenols (TP) in an extract from T. bellerica leaves and fruits, by the UV/Vis spectrophotometric method. The crude extract was used to develop a method for TP assay in T. bellirica. The method was validated according to ICH guidelines. The optimum conditions for analysis time, wavelength and standard substance were 30 min, 760 nm and gallic acid as reference standard, respectively. Under these conditions, validation by UV/Vis spectrophotometry proved the method to be linear, specific, precise, accurate, reproducible, robust, and easy to perform. This methodology complies with the requirements for analytical application and to ensure the reliability of the results.
Keywords: Terminalia bellirica, polyphenols, Folin Ciocalteu method.
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