European Journal of Biomedical and Pharmaceutical Sciences

COMPARISON OF FREQUENCY OF MICRONUCLEI ON LESIONAL AND NORMAL SIDE IN PATIENTS WITH TOBACCO INDUCE KERATOSIS & LEUKOPLAKIA: THE PILOT STUDY

Dr. Sonune Shrikant*, Dr. Patil Ashok, Dr. Kandalgaonkar Shilpa, Dr. Suyog Tupsakhare, Dr. Mahesh Gabhane and Dr. Patil Kishor

ABSTRACT

The assessment of micronuclei in exfoliated cells is a promising tool for the study of the effect of carcinogens on oral epithelium. The technique involve examination of epithelial smears to determine the prevalence of cells containing micronuclei, extra-nuclear bodies composed of chromosomes or chromosomal fragments that failed to be incorporated into daughter nuclei at mitosis. Usually the neoplastic changes take place at the site of chronic stimulus. It passes through various phases of dysplastic changes. Clinically, shows varied spectrum of red and white lesions. Thus it can be hypothesized that micronuclei frequency is higher in that particular lesional tissue compare to normal contra lateral side. Aim of the present study was to compare frequency of micronuclei in Leukoplakia & tobaccos induce keratosis to that of normal contra lateral side. The study consist of 10 patients with clinical diagnosis of Leukoplakia, tobacco induce keratosis. Oral exfoliative cytology from the lesions as well as normal mucosa stained with papanicolaou stain used for counting micronuclei. Result shows that statistically significant difference in frequency of micronuclei on lesional side and non lesional side was found. Perhaps the difference between the frequency of micronuclei on lesional side and normal side almost found to be constant. Thus it can be concluded that, higher frequency of micronuclei suggest the localized effect of carcinogens on the oral mucosa. Perhaps the difference between both sides that is lesional and normal almost remains constant which may be because of the generalized effect of carcinogen on the oral mucosa.

Keywords: Biomarker, Leukoplakia, Micronuclei, Tobacco induced keratosis.