Abstract
AN INTRODUCTION TO INSTRUMENTATION OF ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY

Devika Tripathi*

ABSTRACT

Ultra performance liquid chromatography (UPLC) takes advantage of technological pace made in particle chemistry performance, system optimization, detector design and data processing and control. This new category of analytical separation science retains the practicality and principles of HPLC while creating a step function improvement in chromatographic performance. It uses fine particles and saves time and reduces solvent consumption. The separation on UPLC is performed under very high pressures (up to 100 MPa) without negative influence on analytical column or other components of chromatographic system. It shows a great enhancement in speed, resolution as well as the sensitivity of analysis by using particle size less than 2 μm and the system is designed in a peculiar way to withstand high system back- pressures. This review is basically emphasizes on the basic technology and instrumentation of UPLC.

Keywords: UPLC, HPLC, Sensitivity, Resolution.


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