Abstract
COMPARATIVE STUDY OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITY OF FRACTIONATED PLANT EXTRACTS OF AMLA [PHYLLANTHUS EMBLICA L.], TULSI [OCIMUM TENUIFLORUM L.] AND NEEM [AZADIRACHTA INDICA A.JUSS]

Vedamurthy AB, Padmaa M Paarakh* and Sudisha Jogaiah

ABSTRACT

Fractionated methanol extracts of dried leaves of Phyllanthus emblica, Ocimum tenuiflorum and Azadirachta indica, were evaluated for the antibacterial and antioxidant activity. The extracts were tested for antibacterial activity by spread plate method against Bacillus subtilis and Streptococcus aureus. The antioxidant activity of these fractions were determined by DPPH [1, 1-Diphenyl-2-Picryl Hydrazyl] assay. It was found that gram negative bacteria was largely inhibited by the fraction III of Tulsi than that of Neem and Amla against reference antibacterial drug like Tetracyclin. The zone of inhibition was measured which shows that fraction III of Tulsi is having highest antibacterial activity when compared to Neem and Amla.

Keywords: Neem, Tulsi, Amla, Antibacterial Activity, Ascorbic acid, DPPH.


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