Abstract
BIO ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF AMODIAQUINE AND N-DESETHYL AMODIAQUINE IN HUMAN PLASMA BY LC–MS/MS.

Premanand Ranganathan*, V. Gunasekaran and Indrajeet Singhvi

ABSTRACT

A highly sensitive and simple high performance liquid chromatographic tandem mass spectrometric (LC–MS/MS) assay is developed and validated for the quantification of Amodiaquine and N-Desethyl Amodiaquine in human plasma. Amodiaquine and N-Desethyl Amodiaquine is extracted from human plasma by methyl tertiary butyl ether and analyzed using a reversed phase isocratic elution on a Hypersil Gold (4.6 x 50mm, 4.6μm) column. A acetonitrile: 10mM Ammonium formate with 0.1% Trifluoro acetic acid (75:25 v/v) as mobile phase is used and detection was performed by Ultra triple quadrupole mass spectrometry LC-MS/MS using electrospray ionization in positive mode. Amodiaquine D10 and N- Desethyl Amodiaquine D5 are used as the internal standards. The lower limits of quantification are 0.106 ng/mL and 0.523ng/mL for Amodiaquine and N-Desethyl Amodiaquine respectively. The calibration curves are linear over the concentration range of 0.106 to 51.580 ng/mL for Amodiaquine and 0.523 to 253.855 ng/mL for N-Desethyl Amodiaquine of plasma for each analyte. This novel LC–MS/MS method show satisfactory accuracy and precision and is sufficiently sensitive for the performance of pharmacokinetic studies, Bioavailability studies and Bio Equivalence Studies in humans.

Keywords: Amodiaquine, N-Desethyl Amodiaquine, LC-MS/MS, Human Plasma, Method Validation.


[Full Text Article]   [Download Certificate]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: MAY ISSUE PUBLISHED

    MAY 2024 Issue has been successfully launched on 1 MAY 2024.

  • EJBPS New Impact Factor

    EJBPS Impact Factor has been Increased to 7.482 for Year 2024.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT