SYNTHESIS, CHARACTERIZATION AND ANTI-BACTERIAL ACTIVITY OF SILVER NANOPARTICLES FROM LEAF EXTRACT OF PHYLLANTHUS URINARIA.L.
S. R. Sivakumar*, A. Tamizhazhagan and K. Abdul
ABSTRACT
In present study silver nanoparticles were synthesized from aqueous leaf extract of Indigo era hirsute L. plant leaf extracts will be collected were used and compared for their extracellular of metallic silver nanoparticles. Stable silver nanoparticles formed to be treated aqueous by solution of AgNO3 with the plant leaf extracts as reducing agent Ag-to Ag+. UV spectroscopy is used to monitor the quantitative formation of silver nanoparticles. The synthesized silver nanoparticle is characterized with FT-IR, XRD, SEM and EDAX Characterization by the above said instrument analysis confirmed the presence, size and stability of the silver nanoparticles. After characterization, the silver nanoparticles were tested at various concentrations to check their bactericidal activity against clinical isolates of five bacterial pathogens. The silver nanoparticles exhibited good bactericidal activity at all concentration against all the tested organisms. Maximum zone of inhibition was of observed against Vibrio cholera (18mm at 400g) and minimum level of antibacterial activity was observed against Proteus mirabilis (8mm at 100g). This result suggested the potential use of silver nanoparticles against other clinical pathogens.
Keywords: Phyllanthus urinaria, Silver nanoparticles, poultry pathogens.
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