Abstract
NEW VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF SILYMARIN IN PHARMACEUTICAL FORMULATION

M. Sivanadh* and V. D. N. Kumar Abbaraju

ABSTRACT

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Silymarin in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1ml/min was employed on symmetry Shimadzu LC-20 ATVP Kromasil C-18 column at ambient temperature. The mobile phase consisted of Methanol: Water: Acetonitrile (80:15:5 v/v). The UV detection wavelength was 285nm and 20μl sample was injected. The run time for Silymarin is 15min. The flow rate was found to be 1.5ml/min. The percentage recovery of the method was found to be 100.47%. The LOD and LOQ for Silymarin was found to be 10μg/ml and 40μg/ml respectively. The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation. The HPLC method can be successfully applied for the routine quality control analysis of Silymarin formulations, which could be the better choices compared to the reported methods of literature

Keywords: Silymarin, Rp- HPLC, UV detection, Recovery, Precise.


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