Abstract
NEW VALIDATED RP-HPLC METHOD FOR THE ESTIMATION OF SILYMARIN IN PHARMACEUTICAL FORMULATION

M. Sivanadh* and V. D. N. Kumar Abbaraju

ABSTRACT

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Silymarin in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1ml/min was employed on symmetry Shimadzu LC-20 ATVP Kromasil C-18 column at ambient temperature. The mobile phase consisted of Methanol: Water: Acetonitrile (80:15:5 v/v). The UV detection wavelength was 285nm and 20μl sample was injected. The run time for Silymarin is 15min. The flow rate was found to be 1.5ml/min. The percentage recovery of the method was found to be 100.47%. The LOD and LOQ for Silymarin was found to be 10μg/ml and 40μg/ml respectively. The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation. The HPLC method can be successfully applied for the routine quality control analysis of Silymarin formulations, which could be the better choices compared to the reported methods of literature

Keywords: Silymarin, Rp- HPLC, UV detection, Recovery, Precise.


[Full Text Article]   [Download Certificate]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: MAY ISSUE PUBLISHED

    MAY 2024 Issue has been successfully launched on 1 MAY 2024.

  • EJBPS New Impact Factor

    EJBPS Impact Factor has been Increased to 7.482 for Year 2024.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT