Abstract
STUDY ON SWAB CULTURE VS DEEP TISSSUE BIOPSY CULTURE IN CHRONIC WOUND INFECTIONS

Dr. Selwyn J.Kumar, Prof. Dr. J. A. Jayalal and Dr. Bhagavadh

ABSTRACT

Objectives: Aim of this study is to compare the swab cultures with deep tissue biopsy cultures taken from the
same wounds of diabetic and non diabetic patients with chronic infections. Materials and methods: To compare
the two culture methods, prospective study was conducted in 100 patients admitted with chronic non healing ulcer,
in the surgical septic ward in Kanyakumari Government Medical College Hospital between March 2016 and
December 2016. The patients were divided in to two groups one with patients having diabetes and the other with
non diabetic patients. Both swab and deep tissue biopsy taken from the wounds of these patients and culture and
sensitivity was done on these samples and the results were analysed. Conclusion: Both swab and deep tissue
biopsy techniques have the same results in providing the more comprehensive description of wound flora of both
diabetic and non-diabetic patients in grade 2 and 3 Wagner grade ulcers and in grade 4 and grade 5 ulcer the deep
tissue biopsy will give more specific data.. Infection by Klebsiella and Pseudomonas were the commonest
pathogens. Both the culture method appears valid as long as the wound is cleansed thoroughly and necrotic
material is removed prior to culture collection. It is reasonable to use the swab-based culture method for chronic
non healing wounds.

Keywords: Chronic Wound Infection, Deep Tissue Culture, Swab Culture, Wound Healing.


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