European Journal of Biomedical and Pharmaceutical Sciences

INTERACTION OF RED GRAPE EXTRACT AND NICOTINE IN THE LUNG TISSUE OF MALE ALBINO RAT WITH REFERENCE TO AGING

M. Jayachandrudu, M. Vani, G. Sudhakar, P. Sreenivasulu and K. Chennaiah*

ABSTRACT

Cigarette smoking is the main risk factor for various chronic diseases, including cardiovascular disease, pulmonary disease and cancer. Wine is a widely consumed beverage in the world, with thousands of years of tradition. The phenolic compounds in grape berries are responsible for some of the major organoleptic properties of wine, such as color, astringency, bitterness, and aroma. Age matched rats were be divided into 4 groups of six in each group and treated as follows: Group I: Normal Control (NC) (Control rats received 0.9% saline). Group II: Nicotine treated (Nt) (at a dose of 0.6 mg/ kg body weight by subcutaneous injection for a period of 2 months). Group III: Red grape extract treated (RGEt). (Red grape extract at a doses of 25 mg/ kg body weight via orogastric tube for a period of 2 months). Group IV: Nicotine + Red grape extract treated (Nt+RGEt) (The forth group of rats were received the nicotine + red grape extract as followed by the second and third group). The animals were sacrificed after 24 hours after the last treatment by cervical dislocation and isolated the lung washed with ice-cold saline, immediately immersed in liquid nitrogen and stored at - 800 C for biochemical analysis and enzymatic assays. In the present study the Total Carbohydrates, Glycogen and Total Free Amino Acids, were significantly decreased in nicotine treated rats in lung and increase was observed in the combination treatment (Nt+RGEt), This study suggests red grape extract treatment may be beneficial to young and old age subjects due to nicotine intoxication.

Keywords: Nicotine, Red Grape Extract, Total Carbohydrates, Glycogen, Total Free Amino Acids, Lung and Male Albino Rats.