European Journal of Biomedical and Pharmaceutical Sciences

T-BOOH INDUCED DNA DAMAGE: PROTECTION BY COLEUS AROMATICA

Dinesha Ramadas, Sachidananda Gurumahadevaiah and Subhas Chandrappa Mundasada*

ABSTRACT

Objective: The main objective of the study is to find a natural resource towards preventing oxidative DNA damage induced by t-BOOH. Materials and methods: By using standard antioxidant Butylated Hydroxy Anisole, Coleus aromatica extract, Tertiary butyl hydroperoxide, calf thymus DNA. The sheared DNA was used in submarine agarose gel electrophoresis where DNA damage was done by using tertiary butyl hydroperoxide. Similarly, the cytotoxicity study was also done to know the natural antioxidant itself is not toxic to cells by using lymphocytes and these lymphocytes were isolated from fresh human blood. Results and conclusions: The agarose gel electrophoresis bands were visualized in Transilluminator where, it was confirmed that, tertiary butyl hydroperoxide induced DNA damaged was successfully prevented by BHA (400μM) and Coleus aromatic (25μg) extract. In cell cytotoxicity studies, BHA and Coleus aromatica extract inhibits the cell damage 71% and 75% respectively. The results confirms that, Coleus aromatica extract can inhibit tertiary butyl hydroperoxide induced DNA damage and is nontoxic to cells.

Keywords: Reactive oxygen species, DNA damage, Dietary antioxidant, lymphocytes, t-BOOH.