Abstract
T-BOOH INDUCED DNA DAMAGE: PROTECTION BY COLEUS AROMATICA

Dinesha Ramadas, Sachidananda Gurumahadevaiah and Subhas Chandrappa Mundasada*

ABSTRACT

Objective: The main objective of the study is to find a natural resource towards preventing oxidative DNA damage induced by t-BOOH. Materials and methods: By using standard antioxidant Butylated Hydroxy Anisole, Coleus aromatica extract, Tertiary butyl hydroperoxide, calf thymus DNA. The sheared DNA was used in submarine agarose gel electrophoresis where DNA damage was done by using tertiary butyl hydroperoxide. Similarly, the cytotoxicity study was also done to know the natural antioxidant itself is not toxic to cells by using lymphocytes and these lymphocytes were isolated from fresh human blood. Results and conclusions: The agarose gel electrophoresis bands were visualized in Transilluminator where, it was confirmed that, tertiary butyl hydroperoxide induced DNA damaged was successfully prevented by BHA (400μM) and Coleus aromatic (25μg) extract. In cell cytotoxicity studies, BHA and Coleus aromatica extract inhibits the cell damage 71% and 75% respectively. The results confirms that, Coleus aromatica extract can inhibit tertiary butyl hydroperoxide induced DNA damage and is nontoxic to cells.

Keywords: Reactive oxygen species, DNA damage, Dietary antioxidant, lymphocytes, t-BOOH.


[Full Text Article]   [Download Certificate]

Login





Forgot Password  |  Register

Indexing

Best Paper Awards

European Journal of Biomedical and Pharmaceutical Sciences (EJBPS) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.

Best Article of current issue :

Dr. Dhrubo Jyoti Sen

Download Article : Click here

News & Updation

  • EJBPS: MAY ISSUE PUBLISHED

    MAY 2024 Issue has been successfully launched on 1 MAY 2024.

  • EJBPS New Impact Factor

    EJBPS Impact Factor has been Increased to 7.482 for Year 2024.

  • Index Copernicus Value

    EJBPS Received Index Copernicus Value 77.3, due to High Quality Publication in EJBPS at International Level

  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.

    .

  • Article Invited for Publication

    Dear Researcher, Article Invited for Publication  in EJBPS coming Issue.

     

UG/PG/Ph.D Research Publication

Research Scholar of UG/PG/Ph.D can Submit their Research Article/Review Article/Case Study/Short Communication for Publication in EJBPS

Downloads

Copyright From

Covering Letter

                        Author Instruction 
 

PLAGERLUM REPORT