IN VITRO AND IN SILICO ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIBACTERIAL ACTIVITY OF METHANOL LEAF EXTRACT OF RANDIA SPINOSA
Mahabaleswara K., Chandrasekhar N., Chandrappa C. P., Channabasava and Dr. Govindappa M.*
ABSTRACT
Randia spinosa leaves are using for treating skin diseases and they having important potent bioactive compounds. The methanol leaf extract yielded biologically important compounds viz., glycosides, phytosterol, alkaloids, flavonoids, phenol, tannins, athraquinones. The GC-MS of the same extract yielded nine biologically important compounds. The methanol leaf extract significantly showed strong antioxidant activity in DPPH and FRAP assays. The extract also inhibited the bacteria tested and also strong anti-inflammatory activity by inhibiting the heat induced albumin denaturation and red blood cells membrane stabilization, proteinase inhibition, BSA anti-denaturation and HRBC membrane stabilization. The in silico activity, Pyranol[4,3]benzopyron-1,9-dione, 5a-methoxy-9a-methyl-3-(1-propenyl) perhydro) and benzoic acid, 2-[2-methoxyethoxy)-5 (2,2-dimethylpropanolamine showed the highest binding affinity with high score and good hydrogen bond interactions with active site residues. The activities demonstrated by two compounds suggest that they could be useful in management of oxidant molecules, inflammation and growth of the bacteria.
Keywords: Randia spinosa, phytochemicals, antioxidant, anti-inflammatory, antibacterial, molecular docking.
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