European Journal of Biomedical and Pharmaceutical Sciences

EVALUATION OF ANTIDEPRESSANT ACTIVITY OF ETHANOL AND CHLOROFORM EXTRACTS OF ANTIGONON LEPTOPUS

Poonam Ranjan, Dilip Kumar Tiwari and Kishu Tripathi*

ABSTRACT

The objective of the present study is to investigate the antidepressant activity chloroform and ethanolic extract of leaves of Antigonon leptopusin Albino rat by tail suspension technique. The albino rats weighing about 100-150 gm were used and divided into seven groups, each groups containing six animals (n=6). Control group received distilled water (15 ml), Imipramine HCl (15mg/kg) for standard and test groups received chloroform and ethanolic extract of Antigonon leptopus in five different doses 50mg/kg, 100mg/kg, 150mg/kg, 200mg/kg and 250mg/kg per orally separately. They were evaluated for antidepressant effect by tail suspension model, after 30 minutes for ethanolic extract and after 60 minutes of drug administration. The immobility time was noted for five minutes for each rat in all groups. The results were analyzed by ANOVA followed by Dunnet‟s multiple comparison test, p<0.05 was considered significant. The ethanolic extract at 100mg/kg and 150mg/kg and chloroform extract at 150mg/kg and 200mg/kg exhibited best result in tail suspension method, but the both extract possess significant activity at all test dose level. Present studies were suggested that Antigonon leptopus leaves exhibited good antidepressant properties and this would lead some support for its use in traditional medicine practice.

Keywords: Antigonon leptopus leaves, Albino rat, Tail suspension technique, Antidepressant activity, Chloroform extract, Ethanolic extract.