European Journal of Biomedical and Pharmaceutical Sciences

MUTATION OF INSULIN RECEPTOR SUBSTRATE-1 GENE IN PATIENTS WITH TYPE 2 DIABETES MELLITUS

Dhananjayan R*, Srivani Koundinya KS, Malati T and Vijay Kumar Kutala

ABSTRACT

Type 2 Diabetes Mellitus (T2DM) is the commonest form of diabetes and is characterized by disorders of insulin secretion and insulin resistance, a condition in which cells fail to use insulin properly, sometimes combined with an absolute insulin deficiency. Since, there are not ample studies of association of IRS1 mutation we have done this study. 120 subjects (60 cases and age- and sex- matched 60 controls) were used for the study of IRS 1 mutation. Genomic DNA extraction is done by salting out method. In the Applied Biosystems strategy for automated fluorescent sequencing, fluorescent dye labels are incorporated into DNA extension products using 5´-dye labelled primers (dye primers) or 3´-dye labelled dideoxynucleotide triphosphates (dye terminators). Applied Biosystems DNA sequencers detect fluorescence from four different dyes that are used to identify the A, C, G, and T extension reactions.

Keywords: IRS -1, IMT, Mutation, Diabetes Mellitus, Gene.